Show simple item record

dc.contributor.authorSoo, Alan W
dc.contributor.authorMaher, Belinda M
dc.contributor.authorDaly, Liam
dc.contributor.authorWood, Alfred E
dc.contributor.authorWatson, William R
dc.date.accessioned2011-03-16T15:52:34Z
dc.date.available2011-03-16T15:52:34Z
dc.date.issued2010-11
dc.identifier.citationPreoperative neutrophil response as a predictive marker of clinical outcome following open heart surgery and the impact of leukocyte filtration. 2010, 11 (5):604-11 Interact Cardiovasc Thorac Surgen
dc.identifier.issn1569-9285
dc.identifier.pmid20724426
dc.identifier.doi10.1510/icvts.2009.228056
dc.identifier.urihttp://hdl.handle.net/10147/124889
dc.description.abstractOpen heart surgery is associated with a massive systemic inflammatory response. Neutrophils, are the main mediator of this response. We hypothesised that the degree of neutrophil activation and inflammatory response to open heart surgery varies individually and correlates with clinical outcome. The aim of this study was to determine if individual clinical outcome can be predicted preoperatively through assessment of in-vitro stimulated neutrophil responses. Following that, the effects of neutrophil depletion through leukocyte filters are examined.
dc.description.abstractNeutrophil responses were assessed preoperatively (n=40) through change in neutrophil adhesion molecule [CD11b, CD62L and P Selectin Glycoprotein-1 (PSGL-1)] expression before and after in-vitro stimulation with Phorbol 12-myristate 13-acetate, PMA (1 ng/ml), lipopolysaccharide, LPS (1 μg/ml) and N-Formyl-Met-Leu-Phe, fMLP (1 ng/ml). Stimulated neutrophil responses were then correlated with postoperative clinical outcome. Patients were then randomised to leukocyte filtration (n=20) and a control group (n=20) and the effect of leukocyte filtration on neutrophil response and clinical outcome were investigated.
dc.description.abstractAn individual variation in in-vitro stimulated neutrophil responses was demonstrated. Significant correlations were shown between neutrophil responses and maximum serum creatinine change, CKMB-fraction, adrenaline requirement, noradrenaline requirement, duration of adrenaline required and time to extubation. White cell count and percentage neutrophils were lower in the LD group (P=0.05). CD11b expression (P=0.005) and PSGL-1 expression (P=0.043) across leukocyte filters were also increased. However, no significant difference was detected in clinical outcome between the LD and control groups.
dc.description.abstractPreoperative neutrophil responses to in-vitro stimuli can predict clinical outcome following open heart surgery. However, leukocyte filtration did not offer significant benefit in clinical outcome in our study.
dc.language.isoenen
dc.subject.meshAged
dc.subject.meshAntigens, CD11b
dc.subject.meshCoronary Artery Bypass
dc.subject.meshFemale
dc.subject.meshFlow Cytometry
dc.subject.meshHeart Valve Prosthesis Implantation
dc.subject.meshHumans
dc.subject.meshInflammation
dc.subject.meshL-Selectin
dc.subject.meshLeukapheresis
dc.subject.meshLeukocyte Count
dc.subject.meshLipopolysaccharides
dc.subject.meshMale
dc.subject.meshMembrane Glycoproteins
dc.subject.meshMiddle Aged
dc.subject.meshN-Formylmethionine Leucyl-Phenylalanine
dc.subject.meshNeutrophil Activation
dc.subject.meshNeutrophils
dc.subject.meshPreoperative Period
dc.subject.meshTetradecanoylphorbol Acetate
dc.subject.meshTreatment Outcome
dc.titlePreoperative neutrophil response as a predictive marker of clinical outcome following open heart surgery and the impact of leukocyte filtration.en
dc.typeArticleen
dc.contributor.departmentProf. Eoin O'Malley National Centre for Cardiothoracic Surgery, Mater Misericordiae University Hospital, UCD School of Medicine and Medical Sciences, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Eccle Street, Dublin, Ireland. alan.soo@ucd.ieen
dc.identifier.journalInteractive cardiovascular and thoracic surgeryen
refterms.dateFOA2018-08-22T11:32:42Z
html.description.abstractOpen heart surgery is associated with a massive systemic inflammatory response. Neutrophils, are the main mediator of this response. We hypothesised that the degree of neutrophil activation and inflammatory response to open heart surgery varies individually and correlates with clinical outcome. The aim of this study was to determine if individual clinical outcome can be predicted preoperatively through assessment of in-vitro stimulated neutrophil responses. Following that, the effects of neutrophil depletion through leukocyte filters are examined.
html.description.abstractNeutrophil responses were assessed preoperatively (n=40) through change in neutrophil adhesion molecule [CD11b, CD62L and P Selectin Glycoprotein-1 (PSGL-1)] expression before and after in-vitro stimulation with Phorbol 12-myristate 13-acetate, PMA (1 ng/ml), lipopolysaccharide, LPS (1 μg/ml) and N-Formyl-Met-Leu-Phe, fMLP (1 ng/ml). Stimulated neutrophil responses were then correlated with postoperative clinical outcome. Patients were then randomised to leukocyte filtration (n=20) and a control group (n=20) and the effect of leukocyte filtration on neutrophil response and clinical outcome were investigated.
html.description.abstractAn individual variation in in-vitro stimulated neutrophil responses was demonstrated. Significant correlations were shown between neutrophil responses and maximum serum creatinine change, CKMB-fraction, adrenaline requirement, noradrenaline requirement, duration of adrenaline required and time to extubation. White cell count and percentage neutrophils were lower in the LD group (P=0.05). CD11b expression (P=0.005) and PSGL-1 expression (P=0.043) across leukocyte filters were also increased. However, no significant difference was detected in clinical outcome between the LD and control groups.
html.description.abstractPreoperative neutrophil responses to in-vitro stimuli can predict clinical outcome following open heart surgery. However, leukocyte filtration did not offer significant benefit in clinical outcome in our study.


Files in this item

Thumbnail
Name:
20724426.pdf
Size:
221.4Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record