Contact with existing adipose tissue is inductive for adipogenesis in matrigel.
Authors
Kelly, John LFindlay, Michael W
Knight, Kenneth R
Penington, Anthony
Thompson, Erik W
Messina, Aurora
Morrison, Wayne A
Affiliation
Bernard O'Brien Institute of Microsurgery, Melbourne, Australia.Issue Date
2006-07MeSH
AdipogenesisAdipose Tissue
Animals
Biocompatible Materials
Cell Communication
Cell Differentiation
Collagen
Drug Combinations
Laminin
Mice
Neovascularization, Physiologic
Proteoglycans
Transplantation, Autologous
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Show full item recordCitation
Contact with existing adipose tissue is inductive for adipogenesis in matrigel. 2006, 12 (7):2041-7 Tissue Eng.Publisher
Mary Ann Liebert IncJournal
Tissue engineeringDOI
10.1089/ten.2006.12.2041PubMed ID
16889532Additional Links
http://www.liebertonline.com/doi/abs/10.1089/ten.2006.12.2041Abstract
The effect of adipose tissue on inductive adipogenesis within Matrigel (BD Biosciences) was assessed by using a murine chamber model containing a vascular pedicle. Three-chamber configurations that varied in the access to an adipose tissue source were used, including sealed- and open-chamber groups that had no access and limited access, respectively, to the surrounding adipose tissue, and a sealed-chamber group in which adipose tissue was placed as an autograft. All groups showed neovascularization, but varied in the amount of adipogenesis seen in direct relation to their access to preexisting adipose tissue: open chambers showed strong adipogenesis, whereas the sealed chambers had little or no adipose tissue; adipogenesis was restored in the autograft chamber group that contained 2- to 5-mg fat autografts. These showed significantly more adipogenesis than the sealed chambers with no autograft ( p < 0.01). Autografts with 1mg of fat were capable of producing adipogenesis but did so less consistently than the larger autografts. These findings have important implications for adipose tissue engineering strategies and for understanding de novo production of adipose tissue.Item Type
ArticleLanguage
enISSN
1076-3279ae974a485f413a2113503eed53cd6c53
10.1089/ten.2006.12.2041
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