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    Changes in the metabolic footprint of placental explant-conditioned medium cultured in different oxygen tensions from placentas of small for gestational age and normal pregnancies.

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    Authors
    Horgan, R P
    Broadhurst, D I
    Dunn, W B
    Brown, M
    Heazell, A E P
    Kell, D B
    Baker, P N
    Kenny, L C
    Affiliation
    Department of Obstetrics and Gynaecology, University College Cork, Cork, University Maternity Hospital, The Anu Research Centre, Cork, Ireland., richard.horgan@ucc.ie
    Issue Date
    2012-01-31T16:43:50Z
    MeSH
    Anoxia/*metabolism
    Female
    Humans
    Infant, Newborn
    Infant, Small for Gestational Age/*metabolism
    Metabolomics/methods
    Oxygen/administration & dosage/*metabolism
    Placenta/*metabolism
    Pregnancy
    Principal Component Analysis
    Spectrometry, Mass, Electrospray Ionization
    Tissue Culture Techniques
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    Citation
    Placenta. 2010 Oct;31(10):893-901. Epub 2010 Aug 13.
    Journal
    Placenta
    URI
    http://hdl.handle.net/10147/206254
    DOI
    10.1016/j.placenta.2010.07.002
    PubMed ID
    20708797
    Abstract
    Being born small for gestational age (SGA) confers significantly increased risks of perinatal morbidity and mortality. Accumulating evidence suggests that an SGA fetus results from a poorly perfused and abnormally developed placenta. Some of the placental features seen in SGA, such as abnormal cell turnover and impaired nutrient transport, can be reproduced by culture of placental explants in hypoxic conditions. Metabolic footprinting offers a hypothesis-generating strategy to investigate factors absorbed by and released from this tissue in vitro. Previously, metabolic footprinting of the conditioned culture media has identified differences in placental explants cultured under normoxic and hypoxic conditions and between normal pregnancies and those complicated by pre-eclampsia. In this study we aimed to examine the differences in the metabolic footprint of placental villous explants cultured at different oxygen (O(2)) tensions between women who deliver an SGA baby (n = 9) and those from normal controls (n = 8). Placental villous explants from cases and controls were cultured for 96 h in 1% (hypoxic), 6% (normoxic) and 20% (hyperoxic) O(2). Metabolic footprints were analysed by Ultra Performance Liquid Chromatography coupled to an electrospray hybrid LTQ-Orbitrap Mass Spectrometry (UPLC-MS). 574 metabolite features showed significant difference between SGA and normal at one or more of the oxygen tensions. SGA explant media cultured under hypoxic conditions was observed, on a univariate level, to exhibit the same metabolic signature as controls cultured under normoxic conditions in 49% of the metabolites of interest, suggesting that SGA tissue is acclimatised to hypoxic conditions in vivo. No such behaviour was observed under hyperoxic culture conditions. Glycerophospholipid and tryptophan metabolism were highlighted as areas of particular interest.
    Language
    eng
    ISSN
    1532-3102 (Electronic)
    0143-4004 (Linking)
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.placenta.2010.07.002
    Scopus Count
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    Cork University Maternity Hospital

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    Related articles

    • Changes in the metabolic footprint of placental explant-conditioned culture medium identifies metabolic disturbances related to hypoxia and pre-eclampsia.
    • Authors: Dunn WB, Brown M, Worton SA, Crocker IP, Broadhurst D, Horgan R, Kenny LC, Baker PN, Kell DB, Heazell AE
    • Issue date: 2009 Nov
    • Review: The effects of oxygen on normal and pre-eclamptic placental tissue--insights from metabolomics.
    • Authors: Heazell AE, Brown M, Worton SA, Dunn WB
    • Issue date: 2011 Mar
    • Can oxygen tension contribute to an abnormal placental cytokine milieu?
    • Authors: Peltier MR, Gurzenda EM, Murthy A, Chawala K, Lerner V, Kharode I, Arita Y, Rhodes A, Maari N, Moawad A, Hanna N
    • Issue date: 2011 Oct
    • [Metabolic footprint in conditioned culture medium of placental explants: a comparison between early-onset and late-onset severe preeclampsia].
    • Authors: Liu DY, Chen SL, Wang CH, Luo XM, Huang FF
    • Issue date: 2011 Sep
    • Metabolic profiling uncovers a phenotypic signature of small for gestational age in early pregnancy.
    • Authors: Horgan RP, Broadhurst DI, Walsh SK, Dunn WB, Brown M, Roberts CT, North RA, McCowan LM, Kell DB, Baker PN, Kenny LC
    • Issue date: 2011 Aug 5
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