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dc.contributor.authorKeegan, H
dc.contributor.authorRyan, F
dc.contributor.authorMalkin, A
dc.contributor.authorGriffin, M
dc.contributor.authorLambkin, H
dc.date.accessioned2012-02-01T10:58:09Z
dc.date.available2012-02-01T10:58:09Z
dc.date.issued2012-02-01T10:58:09Z
dc.identifier.citationCytopathology. 2009 Apr;20(2):111-6. Epub 2007 Dec 18.en_GB
dc.identifier.issn1365-2303 (Electronic)en_GB
dc.identifier.issn0956-5507 (Linking)en_GB
dc.identifier.pmid18093220en_GB
dc.identifier.doi10.1111/j.1365-2303.2007.00534.xen_GB
dc.identifier.urihttp://hdl.handle.net/10147/208029
dc.description.abstractOBJECTIVE: The aim of this study was to determine the prevalence of cervical Chlamydia trachomatis infection by polymerase chain reaction (PCR) in urban women undergoing routine cervical cytological screening and to investigate the relationship with age, cytology, smoking status and concurrent human papillomavirus (HPV) infection. METHODS: A total of 996 women (age range 16-69 years) attending general practitioners for routine liquid-based cervical smear screening in the Dublin area were recruited in the study of prevalence of C. trachomatis. Informed consent was obtained and liquid-based cytology (LBC) specimens were sent for cytological screening. DNA was extracted from residual LBC and tested for C. trachomatis by PCR using the highly sensitive C. trachomatis plasmid (CTP) primers and for HPV infection using the MY09/11 primers directed to the HPV L1 gene in a multiplex format. RESULTS: The overall prevalence of C. trachomatis was 5.4%. Prevalence was highest in the <25 years age group (10%). Coinfection with HPV and C. trachomatis occurred in 1% of the screening population. A higher rate of smoking was observed in women positive for C. trachomatis, HPV infections or those with abnormal cervical cytology. Chlamydia trachomatis infection was not associated with abnormal cytology. CONCLUSIONS: Women (5.4%) presenting for routine cervical screening are infected with C. trachomatis. Opportunistic screening for C. trachomatis from PreservCyt sample taken at the time of cervical cytological screening may be a possible strategy to screen for C. trachomatis in the Irish female population.
dc.language.isoengen_GB
dc.subject.meshAdolescenten_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshChlamydia Infections/*diagnosis/epidemiologyen_GB
dc.subject.meshChlamydia trachomatis/*geneticsen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIreland/epidemiologyen_GB
dc.subject.meshMass Screening/methodsen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshSmokingen_GB
dc.subject.mesh*Urban Populationen_GB
dc.subject.mesh*Vaginal Smears/instrumentation/methodsen_GB
dc.subject.meshYoung Adulten_GB
dc.titleChlamydia trachomatis detection in cervical PreservCyt specimens from an Irish urban female population.en_GB
dc.contributor.departmentDepartment of Pathology, Coombe Women's Hospital, Dublin, Ireland., keeganh28@gmail.comen_GB
dc.identifier.journalCytopathology : official journal of the British Society for Clinical Cytologyen_GB
dc.description.provinceLeinster
html.description.abstractOBJECTIVE: The aim of this study was to determine the prevalence of cervical Chlamydia trachomatis infection by polymerase chain reaction (PCR) in urban women undergoing routine cervical cytological screening and to investigate the relationship with age, cytology, smoking status and concurrent human papillomavirus (HPV) infection. METHODS: A total of 996 women (age range 16-69 years) attending general practitioners for routine liquid-based cervical smear screening in the Dublin area were recruited in the study of prevalence of C. trachomatis. Informed consent was obtained and liquid-based cytology (LBC) specimens were sent for cytological screening. DNA was extracted from residual LBC and tested for C. trachomatis by PCR using the highly sensitive C. trachomatis plasmid (CTP) primers and for HPV infection using the MY09/11 primers directed to the HPV L1 gene in a multiplex format. RESULTS: The overall prevalence of C. trachomatis was 5.4%. Prevalence was highest in the <25 years age group (10%). Coinfection with HPV and C. trachomatis occurred in 1% of the screening population. A higher rate of smoking was observed in women positive for C. trachomatis, HPV infections or those with abnormal cervical cytology. Chlamydia trachomatis infection was not associated with abnormal cytology. CONCLUSIONS: Women (5.4%) presenting for routine cervical screening are infected with C. trachomatis. Opportunistic screening for C. trachomatis from PreservCyt sample taken at the time of cervical cytological screening may be a possible strategy to screen for C. trachomatis in the Irish female population.


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