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dc.contributor.authorMcMenamin, Moya
dc.contributor.authorLysakova-Devine, Tatyana
dc.contributor.authorWingfield, Mary
dc.contributor.authorO'Herlihy, Colm
dc.contributor.authorO'Farrelly, Cliona
dc.date.accessioned2013-02-04T14:46:04Z
dc.date.available2013-02-04T14:46:04Z
dc.date.issued2012-09
dc.identifier.citationEndometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells. 2012, 25 (3):322-8 Reprod. Biomed. Onlineen_GB
dc.identifier.issn1472-6491
dc.identifier.pmid22796232
dc.identifier.doi10.1016/j.rbmo.2012.05.001
dc.identifier.urihttp://hdl.handle.net/10147/268232
dc.description.abstractThe aim of this study was to compare the efficacy of endometrial aspiration biopsy (EAB) with the more traditional dilatation and curettage (D&C) for the procurement of lymphoid progenitor cells and uterine natural killer (NK) populations in endometrial tissue. This prospective observational study conducted in a tertiary referral university hospital examined endometrium obtained from 32 women admitted for laparoscopic gynaecological procedures. Each participant had endometrium sampled using both EAB and D&C. Both methods were assessed as a source of uterine NK and lymphoid progenitor cells. Similar proportions of mature CD45+CD56+ NK cells (range 25.4-36.2%) and CD45+CD34+ lymphoid progenitors (range 1.2-2.0%) were found in tissue obtained using both EAB and D&C. These cells were adequate for flow cytometric analysis, magnetic bead separation and culture. Colony formation by the CD34+ population demonstrated maturational potential. Tissues obtained via endometrial biopsy and D&C are equivalent, by analysis of uterine NK and lymphoid progenitor cells. The aim of this study was to compare two methods of endometrial sampling - endometrial aspiration biopsy and traditional dilatation and curettage - for the procurement of haematopoietic stem cells and uterine natural killer (NK) populations in endometrial tissue. Thirty-two women who had gynaecological procedures in a tertiary referral hospital participated in this study and had endometrial tissue collected via both methods. Similar populations of mature NK cells and haematopoietic stem cells were found in tissue obtained using both endometrial aspiration biopsy and dilatation and curettage. Tissue obtained via endometrial aspiration biopsy was adequate for the culture and growth of haematopoietic stem cells. We conclude that tissue obtained via endometrial biopsy and dilatation and curettage is equivalent, by analysis of uterine NK and haematopoietic stem cells using flow cytometry. This has implications for further more extensive study of endometrial haematopoietic stem cell populations, as these samples can be collected in a clinical setting without a requirement for general anaesthesia.
dc.language.isoenen
dc.rightsArchived with thanks to Reproductive biomedicine onlineen_GB
dc.subject.meshAntigens, CD34
dc.subject.meshAntigens, CD45
dc.subject.meshAntigens, CD56
dc.subject.meshBiopsy
dc.subject.meshDilatation and Curettage
dc.subject.meshEndometrium
dc.subject.meshFemale
dc.subject.meshFlow Cytometry
dc.subject.meshHumans
dc.subject.meshKiller Cells, Natural
dc.subject.meshLaparoscopy
dc.subject.meshLymphoid Progenitor Cells
dc.subject.meshProspective Studies
dc.subject.meshStem Cells
dc.subject.meshUterus
dc.titleEndometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells.en_GB
dc.typeArticleen
dc.contributor.departmentUCD School of Medicine and Medical Science, Obstetrics and Gynaecology, University College Dublin, National Maternity Hospital, Dublin 2, Ireland. moyamcmenamin@gmail.comen_GB
dc.identifier.journalReproductive biomedicine onlineen_GB
dc.description.provinceLeinsteren
html.description.abstractThe aim of this study was to compare the efficacy of endometrial aspiration biopsy (EAB) with the more traditional dilatation and curettage (D&C) for the procurement of lymphoid progenitor cells and uterine natural killer (NK) populations in endometrial tissue. This prospective observational study conducted in a tertiary referral university hospital examined endometrium obtained from 32 women admitted for laparoscopic gynaecological procedures. Each participant had endometrium sampled using both EAB and D&C. Both methods were assessed as a source of uterine NK and lymphoid progenitor cells. Similar proportions of mature CD45+CD56+ NK cells (range 25.4-36.2%) and CD45+CD34+ lymphoid progenitors (range 1.2-2.0%) were found in tissue obtained using both EAB and D&C. These cells were adequate for flow cytometric analysis, magnetic bead separation and culture. Colony formation by the CD34+ population demonstrated maturational potential. Tissues obtained via endometrial biopsy and D&C are equivalent, by analysis of uterine NK and lymphoid progenitor cells. The aim of this study was to compare two methods of endometrial sampling - endometrial aspiration biopsy and traditional dilatation and curettage - for the procurement of haematopoietic stem cells and uterine natural killer (NK) populations in endometrial tissue. Thirty-two women who had gynaecological procedures in a tertiary referral hospital participated in this study and had endometrial tissue collected via both methods. Similar populations of mature NK cells and haematopoietic stem cells were found in tissue obtained using both endometrial aspiration biopsy and dilatation and curettage. Tissue obtained via endometrial aspiration biopsy was adequate for the culture and growth of haematopoietic stem cells. We conclude that tissue obtained via endometrial biopsy and dilatation and curettage is equivalent, by analysis of uterine NK and haematopoietic stem cells using flow cytometry. This has implications for further more extensive study of endometrial haematopoietic stem cell populations, as these samples can be collected in a clinical setting without a requirement for general anaesthesia.


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