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dc.contributor.authorFanning, Rebecca A
dc.contributor.authorMcMorrow, Jason P
dc.contributor.authorCampion, Deirdre P
dc.contributor.authorCarey, Michael F
dc.contributor.authorO'Connor, John J
dc.date.accessioned2013-06-26T08:50:24Z
dc.date.available2013-06-26T08:50:24Z
dc.date.issued2013-01-05
dc.identifier.citationOpioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium. 2013, 698 (1-3):170-7 Eur. J. Pharmacol.en_GB
dc.identifier.issn1879-0712
dc.identifier.pmid23051674
dc.identifier.doi10.1016/j.ejphar.2012.09.045
dc.identifier.urihttp://hdl.handle.net/10147/294576
dc.description.abstractThe existence of opioid receptors in mammalian myometrial tissue is now widely accepted. Previously enkephalin degrading enzymes have been shown to be elevated in pregnant rat uterus and a met-enkephalin analogue has been shown to alter spontaneous contractility of rat myometrium. Here we have undertaken studies to determine the effects of met-enkephalin on in vitro human myometrial contractility and investigate the expression of opioid receptors in pregnant myometrium. Myometrial biopsies were taken from women undergoing elective caesarean delivery at term. Organ bath experiments were used to investigate the effect of the met-enkephalin analogue [d-Ala 2, d-met 5] enkephalin (DAMEA) on spontaneous contractility. A confocal immunofluorescent technique and real time PCR were used to determine the expression of protein and mRNA, respectively for two opioid receptor subtypes, mu and delta. DAMEA had a concentration dependent inhibitory effect on contractile activity (1 × 10(-7)M-1 × 10(-4)M; 54% reduction in contractile activity, P<0.001 at 1 × 10(-4)M concentration). Mu and delta opioid receptor protein sub-types and their respective mRNA were identified in all tissues sampled. This is the first report of opioid receptor expression and of an opioid mediated uterorelaxant action in term human non-labouring myometrium in vitro.
dc.language.isoenen
dc.publisherElsevieren_GB
dc.rightsArchived with thanks to European journal of pharmacologyen_GB
dc.subject.meshAdult
dc.subject.meshAnalgesics, Opioid
dc.subject.meshEnkephalin, Methionine
dc.subject.meshFemale
dc.subject.meshGene Expression Regulation
dc.subject.meshHumans
dc.subject.meshMyometrium
dc.subject.meshPregnancy
dc.subject.meshReceptors, Opioid, delta
dc.subject.meshReceptors, Opioid, mu
dc.subject.meshTerm Birth
dc.subject.meshUterine Contraction
dc.subject.meshYoung Adult
dc.titleOpioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium.en_GB
dc.typeArticleen
dc.contributor.departmentDepartment of Perioperative Medicine, Coombe Women and Infants University Hospital, Cork Street, Dublin 8, Ireland.en_GB
dc.identifier.journalEuropean journal of pharmacologyen_GB
dc.description.provinceLeinsteren
html.description.abstractThe existence of opioid receptors in mammalian myometrial tissue is now widely accepted. Previously enkephalin degrading enzymes have been shown to be elevated in pregnant rat uterus and a met-enkephalin analogue has been shown to alter spontaneous contractility of rat myometrium. Here we have undertaken studies to determine the effects of met-enkephalin on in vitro human myometrial contractility and investigate the expression of opioid receptors in pregnant myometrium. Myometrial biopsies were taken from women undergoing elective caesarean delivery at term. Organ bath experiments were used to investigate the effect of the met-enkephalin analogue [d-Ala 2, d-met 5] enkephalin (DAMEA) on spontaneous contractility. A confocal immunofluorescent technique and real time PCR were used to determine the expression of protein and mRNA, respectively for two opioid receptor subtypes, mu and delta. DAMEA had a concentration dependent inhibitory effect on contractile activity (1 × 10(-7)M-1 × 10(-4)M; 54% reduction in contractile activity, P<0.001 at 1 × 10(-4)M concentration). Mu and delta opioid receptor protein sub-types and their respective mRNA were identified in all tissues sampled. This is the first report of opioid receptor expression and of an opioid mediated uterorelaxant action in term human non-labouring myometrium in vitro.


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