A Low Glycaemic Index Diet in Pregnancy Induces DNA Methylation Variation in Blood of Newborns: Results from the ROLO Randomised Controlled Trial.
AuthorsGeraghty, Aisling A
O'Brien, Eileen C
McAuliffe, Fionnuala M
AffiliationNational Maternity Hospital Holles Street Dublin, University College Dublin, Cancer and Disease Epigenetics, Murdoch Children's Research Institute, Melbourne, Victoria, Aus, Department of Paediatrics, University of Melbourne, Victoria, Aus
MetadataShow full item record
CitationGeraghty, A.A.; Sexton-Oates, A.; O’Brien, E.C.; Alberdi, G.; Fransquet, P.; Saffery, R.; McAuliffe, F.M. A Low Glycaemic Index Diet in Pregnancy Induces DNA Methylation Variation in Blood of Newborns: Results from the ROLO Randomised Controlled Trial. Nutrients 2018, 10, 455.
AbstractThe epigenetic profile of the developing fetus is sensitive to environmental influence. Maternal diet has been shown to influence DNA methylation patterns in offspring, but research in humans is limited. We investigated the impact of a low glycaemic index dietary intervention during pregnancy on offspring DNA methylation patterns using a genome-wide methylation approach. Sixty neonates were selected from the ROLO (Randomised cOntrol trial of LOw glycaemic index diet to prevent macrosomia) study: 30 neonates from the low glycaemic index intervention arm and 30 from the control, whose mothers received no specific dietary advice. DNA methylation was investigated in 771,484 CpG sites in free DNA from cord blood serum. Principal component analysis and linear regression were carried out comparing the intervention and control groups. Gene clustering and pathway analysis were also explored. Widespread variation was identified in the newborns exposed to the dietary intervention, accounting for 11% of the total level of DNA methylation variation within the dataset. No association was found with maternal early-pregnancy body mass index (BMI), infant sex, or birthweight. Pathway analysis identified common influences of the intervention on gene clusters plausibly linked to pathways targeted by the intervention, including cardiac and immune functioning. Analysis in 60 additional samples from the ROLO study failed to replicate the original findings. Using a modest-sized discovery sample, we identified preliminary evidence of differential methylation in progeny of mothers exposed to a dietary intervention during pregnancy.
SponsorsThis study was supported by the Health Research Board Ireland, Health Research Centre for Health and Diet Research, The National Maternity Hospital Medical Fund, and the European Union’s Seventh Framework Programme (FP7/2007-2013), project EarlyNutrition under grant agreement No. 289346. Richard Saffery is supported by a National Health and Medical Research Council (Australia) Senior Research Fellowship and the Victorian Government Operational Infrastructure support scheme.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States
- Epigenetic Patterns in Five-Year-Old Children Exposed to a Low Glycemic Index Dietary Intervention during Pregnancy: Results from the ROLO Kids Study.
- Authors: Geraghty AA, Sexton-Oates A, O'Brien EC, Saffery R, McAuliffe FM
- Issue date: 2020 Nov 24
- Maternal low glycaemic index diet, fat intake and postprandial glucose influences neonatal adiposity--secondary analysis from the ROLO study.
- Authors: Horan MK, McGowan CA, Gibney ER, Donnelly JM, McAuliffe FM
- Issue date: 2014 Aug 1
- Low glycaemic index diet in pregnancy to prevent macrosomia (ROLO study): randomised control trial.
- Authors: Walsh JM, McGowan CA, Mahony R, Foley ME, McAuliffe FM
- Issue date: 2012 Aug 30
- Maternal Nutrition and Glycaemic Index during Pregnancy Impacts on Offspring Adiposity at 6 Months of Age--Analysis from the ROLO Randomised Controlled Trial.
- Authors: Horan MK, McGowan CA, Gibney ER, Byrne J, Donnelly JM, McAuliffe FM
- Issue date: 2016 Jan 4
- Impact of maternal diet on neonatal anthropometry: a randomized controlled trial.
- Authors: Donnelly JM, Walsh JM, Byrne J, Molloy EJ, McAuliffe FM
- Issue date: 2015 Feb