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    Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.

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    Authors
    Creagh, Emma M
    Brumatti, Gabriela
    Sheridan, Clare
    Duriez, Patrick J
    Taylor, Rebecca C
    Cullen, Sean P
    Adrain, Colin
    Martin, Seamus J
    Affiliation
    Molecular Cell Biology Laboratory, Department of Genetics, Smurfit Institute, Trinity College, Dublin, Ireland [corrected]
    Issue Date
    2009
    MeSH
    Animals
    Apoptosis
    Caspases
    Cell Line
    Cell Survival
    Drosophila
    Drosophila Proteins
    Humans
    Mammals
    Proteomics
    RNA-Binding Proteins
    Species Specificity
    Substrate Specificity
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    Citation
    Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival. 2009, 4 (3):e5055 PLoS ONE
    Journal
    PloS one
    URI
    http://hdl.handle.net/10147/94731
    DOI
    10.1371/journal.pone.0005055
    PubMed ID
    19330035
    Abstract
    Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.
    Language
    en
    ISSN
    1932-6203
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0005055
    Scopus Count
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